Figure 1
EPR spectra of the •NO-Fe(MGD) 2 spin adduct formed from the release of •NO from tobacco xenobiotics in phosphate buffered-saline at pH 7.4 (left column) and human saliva (right column). The hyperfine splitting value, aN, of these spectra is 12.4 G and the isotropic nuclear g value, giso, is 2.04. (a) 500 μL phosphate buffered saline (left column) or whole human saliva (right column); (b) 500 μL of a 1:1 w/v extract of smokeless tobacco; (c) 3.48 M (26.7 μG) nicotine; (d) 1.1 M (10 mg) nitrosonornicotine (NNN); and (e) 1.0 M (10 mg) 4-(methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone (NNK). Each spectrum represents 15 summed signal acquisitions acquired using a receiver gain of 2 × 104 and modulation amplitude of 1 G. The ordinate scale is ± 1 × 103 arbitrary units for all spectra in whole human saliva.