Tobacco Xenobiotics Release Nitric Oxide

Table 1 Chemiluminescent detection of ^•NO

	Total ^•NO observed
	in PBS (pH 7.4)	in human saliva
PBS	5 ± 1 μM^a	-
Whole human saliva (WHS)	-	38 ± 17 μM^a
Smokeless tobacco (ST)	1100 ± 50 μM^a 2.53 ± 0.10 μmol/g ST	1380 ± 80 μM^a 2.76 ± 0.16 μmol/g ST
Nicotine	< 0.02 μM^b	150 ± 12 μM^a 2.81 ± 0.23 nmol/mg nicotine
Nitrosonornicotine (NNN)	< 0.02 μM^b	121 ± 6 μM^a .90 ± 0.30 nmol/mg NNN
4-(Methyl-N-nitrosamino)-1-(3- pyridyl)-1-butanone (NNK)	< 0.02 μM^b	113 ± 5 μM^a 5.45 ± 0.25 nmol/mg NNK

^aThis is the apparent concentration of ^•NO present as NO₂^- in the aqueous incubation injected into the Sievers NOA analyzer.
^bThe limit of detection with our experimental conditions.

ISSN: 1617-9625