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Figure 1 | Tobacco Induced Diseases

Figure 1

From: Effect of nicotine on exocytotic pancreatic secretory response: role of calcium signaling

Figure 1

Primary cells were washed, incubated at 37°C with 100 μM nicotine for 0 to 10 minutes (Panel A) or at various concentrations of nicotine (Panel B) and then incubated with CCK-8 (10-9) M, or without CCK-8 for 30 minutes. Amylase released into the medium was measured with Procion-yellow starch as substrate. The data are presented as % initial content and represented as the mean ± SEM of four separate experiments (unstimulated, circles with solid line); (stimulated, triangles with dashed line).

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