Figure 3

Effect of H-7 or 2-APB on primary cell function, with or without nicotine. Panel A: Primary cells (4–6 x 10⁶) were washed and incubated at 37°C with or without 10 μM H-7 for 30 minutes, followed by an additional incubation with nicotine for 6 minutes. Cells were washed and then incubated at 37°C with or without CCK-8 (10^-9 M) for 30 minutes. Amylase released into the incubation medium was measured with Procion-yellow starch as substrate. The data are presented as % initial content and represented as the mean ± SEM of four experiments. Panel B: Primary cells (4–6 x 10⁶) were washed and incubated at 37°C without or with 80 mM 2-APB for 30 minutes, followed by an additional 6 minutes incubation with nicotine. Amylase released into the incubation medium was measured with Procion-yellow starch as substrate. The data are presented as % initial content and represented as the mean ± SEM of four experiments. *P < .05 between control and nicotine-added samples; **P < .05 between nicotine-added and H-7 + nicotine-added or 2-APB + nicotine added samples.