Table 1 Quantitative RT-PCR primer sequences used in this study
Primers | Sequences | Melting temp | Product size | Reference |
|---|---|---|---|---|
HBD1 Forward | 5′-CCCAGTTCCTGAAATCCTGA-3′ | 56°C | 215bp | (Han et al., 2008) [15] |
Reverse | 5′-CAGGTGCCTTGAATTTTGGT-3′ | 56°C | ||
HBD3 Forward | 5′-AGCCTAGCAGCTATGAGGATC-3′ | 56°C | 206bp | (Han et al., 2008) [15] |
Reverse | 5′-CTTCGGCAGCATTTTGCGCCA-3′ | |||
HBD5 Forward | 5′-TCCATCAGGTGAGTTTGCTG-3′ | 57°C | 105bp | (*) |
Reverse | 5′-GTTCAGCCTGCAATTTCCAT-3′ | |||
HBD8 Forward | 5′-TGCCTTGAAACAGAAATCCA-3′ | 56°C | 104bp | (**) |
Reverse | 5′-TCCTTTTTGGGTGTAGTGCTC -3′ | |||
HBD9 Forward | 5′-GGCCTAAATCCAGGTGTGAA-3′ | 57°C | 174bp | (Alekseeva et al., 2009) [33] |
Reverse | 5′-TCAAATGTTGGCAAGTGGAG-3′ | |||
HBD18 Forward | 5'-TGCATTCCATCCAATGAAGA-3′ | 57°C | 181bp | (***) |
Reverse | 5′-GAGGTCTCAGTTCCCCTTCC-3′ | |||
LL-37 Forward | 5′-CTAGAGGGAGGCAGACATGG-3′ | 57°C | 201bp | (Amer, Bishop, & van Hoek, 2010) [32] |
Reverse | 5′-AGGAGGCGGTAAGGTTAGC-3′ |