Figure 7

Western blot analysis of caspase-3 from lysates of isolated fetal rat lung fibroblasts exposed to different concentrations of CSE. Fetal rat lung fibroblasts were isolated on the 21st day of gestation and grown to 70–80% confluence in an incubator at 37°C. The cells were exposed to different concentrations of CSE (5%, 10% and 15%) (v/v) in serum free media for 60 min keeping controls which were not exposed to CSE. (A) Lane 1: cell lysates not exposed to CSE. Lane 2: cell lysates exposed to 5% CSE. Lane 3: Cell lysates exposed to 10% CSE. Lane 4: Cell lysates exposed to 15% CSE. (B) Densitometric analysis of band intensity shows each bar represents the mean ± SEM of three experiments. (*) indicates significantly different from the corresponding controls (p < 0.05). A β-actin loading control is shown beneath the plot.