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Figure 9 | Tobacco Induced Diseases

Figure 9

From: Caspase 3 activity in isolated fetal rat lung fibroblasts and rat periodontal ligament fibroblasts: cigarette smoke induced alterations

Figure 9

Immunofluorescence staining for detection of caspase 3 expression in isolated fetal rat lung fibroblasts. Isolated fetal rat lung fibroblasts were exposed to CSE (15% v/v) for three hours. Immunofluorescence was performed using caspase 3 rabbit polyclonal IGg antibody. Caspase 3 was visualized using donkey anti-rabbit IGg-FITC (green fluorescence) and counter stained with Hoescht 33342 (nuclear staining - blue). Image (A) shows controls not exposed to CSE. Image (B) shows expression of caspase 3 (green) primarily localized in the cytoplasm. Image (C) shows an enlarged image of a single cell.

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