Figure 3

Dose and time dependent induction of ERK1/2 in AR42J cell s. Cell lysates were loaded onto an SDS gel, separated by electrophoresis, blocked in 1% fat free milk and probed with antibodies to total and phosphorylated (p) ERK1/2. Horseradish peroxidase-coupled anti IgG was used as a secondary antibody. Bands were visualized with ECL-plus and quantified using a STORM Imager. The data shown as means ± SEM of n = 5 experiments. A: Induction of ERK1/2 in cells exposed to 20 μM H₂O₂ for 10–60 min compared to control untreated cells. B: Induction of ERK in cells exposed to 10–20 μM H₂O₂ for 15 min. C: Band intensity showing the fold increase in the time dependent induction of ERK1/2. D: Band intensity showing the fold increase in the dose dependent induction of ERK1/2.