Figure 4

Induction of ERK1/2 in AR42J cells exposed to nicotine or H ₂ O ₂ . Control untreated cells, cells exposed to 20 μM H₂O₂ for 15 min or 100 μM nicotine 3 min were lysed and the lysates were used for Western blotting. A: Western blot visualization with ECL-plus using STORM Imaging software. B: Band intensity showing the fold increase as mean ± SEM of n = 5 experiments. *, P < 0.05, significantly different from the uncxposed control.