Figure 6

The effect of nicotine or hydrogen peroxide on the proliferation of AR42J cells. The cells were plated in 96-well plates and allowed to attach overnight before transferring to 0.05% serum media for 10–12 h before beginning of the study. The cells were then treated with 100 μM nicotine or 20 μM H₂O₂ and the proliferation measured at 24–96 h using a cell counting kit from Dojindo Molecular Technologies according to manufacturer's instructions. The data points represent mean ± SEM of 5 experiments. ◆,-- control unexposed cells; ■, --- Nicotine treated; ▲ --, H₂O₂ treated. *, P < 0.05, significantly different from the uncxposed control.