Figure 7

Comparison of cell function of AR42J cells when exposed to nicotine or H₂O₂. The cells were treated with 100 μM nicotine for 3 min or 20 μM H₂O₂ 15 min. Cells were subsequently washed with Hepes-Ringer and incubated at 37°C with or without CCK-8 (10 nM) for 30 min. Amylase released into the incubation medium was measured with procion yellow starch as a substrate. The data are expressed as percent initial content and represent mean ± SEM of 5 experiments. open bar---control unexposed; hatched bar---- CCK-8 stimulated; *, p < 0.05, significantly different from the control; **, p < 0.05, significantly different from H₂O₂ exposed cells.