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Figure 6 | Tobacco Induced Diseases

Figure 6

From: Effects of whole cigarette smoke on human beta defensins expression and secretion by oral mucosal epithelial cells

Figure 6

Subcellular localization of HBD-1, -2 and -3 in Leuk-1 cells. A. HBD-1 (green) was mainly localized in perinuclear cytoplasm and nuclei. Reticular immunostaining of HBD-1 was clearly observed in perinuclear cytoplasm (asterisk) and strong expression of HBD-1 was especially noted in nucleoli area (closed arrowhead). Following 40 min-WCS exposure, reticular immunostaining of HBD-1 in perinuclear cytoplasm nearly disappeared and the expression of HBD-1 in nucleoli area was weak (closed arrowhead). B. The weak expression of HBD-2 (red) was mainly in perinuclear cytoplasm. Some expression of HBD-2 could also be detected in cytoplasmic vesicles (arrow). Following 40 min-WCS exposure, the expression of HBD-2 dramatically enhanced and localized in perinuclear cytoplasm. C. HBD-3 (red) was mainly localized in perinuclear cytoplasm and nucleoli area (open arrowhead), which was expressed weakly. Following 40 min-WCS exposure, the expression of HBD-3 dramatically augmented and still localized in perinuclear cytoplasm and nucleoli area (open arrowhead). Scale bar = 20 μm.

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Tobacco Induced Diseases

ISSN: 1617-9625

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